Qubit vs Nanodrop discrepancies??

[amylee86]

Hi all,

What kind of difference is expected when quantifying dsDNA on a nanodrop vs. Qubit?

Our core facility has generated Qubit values for my cDNA sampels that are consistently 1.3- to 2.3-fold higher than the Nanodrop values that I generated. I've read a lot of reports claiming that Nanodrop consistently overestimates concentration, so I'm a little puzzled by these Qubit results.

Any help is greatly appreciated!

Thanks in advance,
Amy

[LB2301]

I have this same problem with my samples!! you managed to solve your doubts? please if your answer is positive, I discuss how? I have this same problem and I think that may be the differences in temperature between rooms!!

thank you