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  • Himalaya
    Member
    • Jun 2010
    • 38
    #1

    newbler quality trimming

    Hi All
    Does anyone has tried to do the quality trimming of 454 short sequence reads? My 454 short sequence reads are already trimmed for adaptors and MIDs. But there are poor quality bases that I wanted to trim. Anyone please help me out to do quality trim with Newbler...

    Himalaya
    Tags: 454reads, newbler, quality, short sequence, trimming
    • flxlex
      Moderator
      • Nov 2008
      • 412
      #2
      Newbler will automatically trim off low quality bases for you. If you really want to do it yourself, you could use several tools, e.g. PRINSEQ, http://edwards.sdsu.edu/prinseq_beta/#

        Comment

        • Himalaya
          Member
          • Jun 2010
          • 38
          #3
          I found another tool QTrim for quality trimming. (hiv.sanbi.ac.za/software/qtrim)

            Comment

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