Hi!
could you upload this technical bulletin again?
I cannot download it from the link you provided already a decade ago...
Thanks!
Ellli
-
please give me this technical bulletin
Dear sir, can you give me this technical bulletin?thank you so much.Originally posted by Vartul View PostLeave a comment:
-
Roche 454 RL adapters.. help!!!
Hi,
We have prepared a paired end library for 454 GS junior but now would like to try rapid library protocol on a couple of isolates. We have all the reagents needed for the rapid library preparation except for adaptors. I asked Roche but they do not supply adaptors separately . We do not want to multiplex several libraries therefore, a MID adaptor kit would be useless. It will also cost a fortune as will cost a new RL library preparation kit. We have noticed that IDT (http://eu.idtdna.com/order/seqset.aspx?type=rapid) can synthesize a set of (two) adaptors. I would request for an advice if a set of adaptors synthesized by IDT can be used instead of Roche adaptors? If yes, what should be the concentration of these oligos in the ligation reaction?
Roche supplies a single tube with Y shaped adaptors and according to their protocol, we just need to add 1ul to the reaction.
Thank you,
Vartul
-
The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...08-27-2024, 04:44 AM -
Sequencing mRNA provides a snapshot of cellular activity, allowing researchers to study the dynamics of cellular processes, compare gene expression across different tissue types, and gain insights into the mechanisms of complex diseases. “mRNA’s central role in the dogma of molecular biology makes it a logical and relevant focus for transcriptomic studies,” stated Sebastian Aguilar Pierlé, Ph.D., Application Development Lead at Inorevia. “One of the major hurdles for...08-07-2024, 12:11 PM
Leave a comment: