Hi!
could you upload this technical bulletin again?
I cannot download it from the link you provided already a decade ago...
Thanks!
Ellli
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please give me this technical bulletin
Dear sir, can you give me this technical bulletin?thank you so much.Originally posted by Vartul View PostLeave a comment:
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Roche 454 RL adapters.. help!!!
Hi,
We have prepared a paired end library for 454 GS junior but now would like to try rapid library protocol on a couple of isolates. We have all the reagents needed for the rapid library preparation except for adaptors. I asked Roche but they do not supply adaptors separately . We do not want to multiplex several libraries therefore, a MID adaptor kit would be useless. It will also cost a fortune as will cost a new RL library preparation kit. We have noticed that IDT (http://eu.idtdna.com/order/seqset.aspx?type=rapid) can synthesize a set of (two) adaptors. I would request for an advice if a set of adaptors synthesized by IDT can be used instead of Roche adaptors? If yes, what should be the concentration of these oligos in the ligation reaction?
Roche supplies a single tube with Y shaped adaptors and according to their protocol, we just need to add 1ul to the reaction.
Thank you,
Vartul
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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