Hey folks.
I'm attempting to do a 454 Junior run with 54 pooled libraries, and it seems that there are only 14 suggested MIDs for use with the GS Junior chemistry. Supposedly these are optimised for easy differentiation, but theoretically you can create your own additional tags as well. Does anybody have success with homebrewed MID tags and care to share some sequences? Or is there a way to combine multiple tags? Some of my amplicons could be slightly larger than the available read length, so putting an MID on each end might not work.
Any feedback is appreciated!
I'm attempting to do a 454 Junior run with 54 pooled libraries, and it seems that there are only 14 suggested MIDs for use with the GS Junior chemistry. Supposedly these are optimised for easy differentiation, but theoretically you can create your own additional tags as well. Does anybody have success with homebrewed MID tags and care to share some sequences? Or is there a way to combine multiple tags? Some of my amplicons could be slightly larger than the available read length, so putting an MID on each end might not work.
Any feedback is appreciated!
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