Hi all!
Can't find the adaptors sequence for Amplicon Sequencing)) Can smb help me?
thanks!)))
Can't find the adaptors sequence for Amplicon Sequencing)) Can smb help me?
thanks!)))
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maybe you should start specifying which technology you're using on which instrument... -
Ion Torrent, Amplicon sequencing))Comment
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Assuming it's Ion Torrent as it's in the Ion Torrent thread...Comment
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In this manual are only the primer sequence, ... but adaptor sequence absence? is they full complementary and same size?Last edited by Chartiza; 02-01-2012, 03:53 AM.Comment
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The PCR creates the adapter sequences using the P1 and A tags on the site specific primers.Comment
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having the sequence of the (Y) adaptors would be handy!Comment
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What's interesting is that these sequences seem to be a mash up of the 454 and Solid sequences. There is even some part complimentary sequence between the forward and reverse primer sequences. Surely this is would promote primer-dimer?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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