Has anyone here used the Opentrons OT-2 for library preparation? https://opentrons.com/ot-2
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"Accuracy Accurate down to 384-well plates"
This is a new measure of accuracy that I was previously unaware of.
Their only 'Sequencing Application' in their protocol library is for 16S. There are liquid handling platforms with a much wider array of NGS protocols ready to go than this.. -
A neighboring lab has the OT-One. This first version is unusable for just about anything - it can't hold the pipettes properly and the plates have a lot of play. Version two looks very different, though.Comment
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There have recently been a few discussions about them on Reddit; perhaps it might be a way to get in touch with people who use them.Comment
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We recently got an OT2. So far it works for single channel pipetting.
We have problems with both of our multi-channel pipettes.
The magnetic module seems to have a design flaw - the magnet is too close to the samples, even when disengaged and at maximum distance (achieved with an self-made adapter). Thus, the beads do not stay in suspension for long - ideally they should stay in suspension for 5 minutes before then engaging the magnet.Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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