Nanopore: Input cDNA Length vs. Read Length?

[JVGen]

Hi All,

I'm trying to get an idea of the processivity of Nanopore. For instance, do the pores reliably read the full length of 5 kb DNA molecules, or do they dissociate from the pore before completing? I want to be sure there isn't a substantial negative bias toward long DNA molecules, because those are of interest in my experiment. Seems like comparing the length distribution of the cDNA input to the read length distribution would address this question, but I'm having trouble finding reports/publications that include the distribution of the cDNA library.

For those of you with hands on experience, do you find that the length of cDNA libraries agree with the length of the output reads?

TYIA!

[fchatonnet]

Dear JVGen,
for all I know about Nanopore sequencing, it should be able to sequence even the longest cDNAs.
Maybe you can find something useful here: https://www.biorxiv.org/content/10.1...892v1.full.pdf

Good luck!