Has anybody tried the TruSeq small RNA sample prep kit to prepare libraries for directional mRNA sequencing? We would like to multiplex some bacterial samples, and this kit should work if we fragment the RNA first.
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Pair end adapter stripping
I am using small RNA Truseq kit for pair end directional RNA seq. I am just wondering if somebody knows what adapters' sequence I can use to strip the 3' and 5' adapters. My fragments are shorter than the read length and it is important to strip off the two adapters. Thanks! -
Has anyone added the Parkhomchuck UNG step to TruSeq RNA?Comment
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Yes. I've done about 20 samples using Parkhomchuck mods with TruSeq RNA reagents and it works pretty well. I get about 93% of the reads in the correct direction.Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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