Hi!
We are doing Small-RNAseq libraries since a little bit (using TruSeq Small RNA prep kit) and we have problem quantifying the libraries accurately. We are using the KAPA quantification kit, but we have very variable read counts on the HiSeq with this technique. We suspect that KAPA quantification kit is not optimal for quantifying this kind of libraries since the standards are longer than the library fragments.
What quantification technique do you guys use for Small-RNAseq?
Thanks!
We are doing Small-RNAseq libraries since a little bit (using TruSeq Small RNA prep kit) and we have problem quantifying the libraries accurately. We are using the KAPA quantification kit, but we have very variable read counts on the HiSeq with this technique. We suspect that KAPA quantification kit is not optimal for quantifying this kind of libraries since the standards are longer than the library fragments.
What quantification technique do you guys use for Small-RNAseq?
Thanks!
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