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I want to do chip-seq for a transcription factor in bacteria for Illumina. I' need help for sample preparation for bacteria since all I find is eukaryotes. Brand new with the technique, sorry!
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I haven't done ChIP in bacteria but once you have the ChIP material I would think making the libraries would be the same. I'm waiting to get back sequencing data from my first ChIP seq libraries. If all looks good then I will be able to give some advice. You could contact Illumina to see if they have any protocols for ChIP in prokayotes. -
A very interesting study shows some of the pitfalls of ChIP applications and offers some advances to published protocols.
The paper is dedicated to ChIP on Chip but most of it also applies to Chip-Seq, too.
If you're new to the tech, I would recommend reading this paper.
Here's the ref: Waldminghaus & Skarstad 2010, BMC Genomics, 11:414Comment
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Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
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Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...07-08-2026, 05:17 AM -
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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