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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by cub103, 07-28-2010, 12:12 PM
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3 responses
2,385 views
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by josdegraaf
08-02-2010, 06:26 AM
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Started by hanjaylee, 12-23-2009, 03:02 AM
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15 responses
7,125 views
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by kainsteven
07-30-2010, 09:21 AM
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Started by icanper, 07-28-2010, 02:38 AM
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2 responses
4,337 views
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by nasobema
07-30-2010, 02:34 AM
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Started by lindenb, 07-29-2010, 07:18 AM
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3 responses
3,619 views
0 reactions
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by adamdeluca
07-29-2010, 11:59 AM
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Started by mestro2, 07-05-2010, 04:34 AM
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10 responses
5,994 views
0 reactions
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Last Post
by upenn_ngs
07-28-2010, 08:27 AM
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Started by obsidiana, 07-27-2010, 02:40 AM
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6 responses
3,589 views
0 reactions
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Last Post
by obsidiana
07-28-2010, 06:17 AM
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Started by MGH Man, 01-11-2010, 03:08 PM
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5 responses
7,856 views
0 reactions
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by JUdw
07-26-2010, 05:15 AM
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Started by HGENETIC, 07-26-2010, 01:44 AM
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0 responses
2,295 views
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by HGENETIC
07-26-2010, 01:44 AM
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Started by NextGenSeq, 07-14-2010, 01:33 PM
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2 responses
3,997 views
0 reactions
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Last Post
by NextGenSeq
07-22-2010, 06:39 AM
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Started by nedoluzhko, 07-19-2010, 07:32 AM
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1 response
3,402 views
0 reactions
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Last Post
by snetmcom
07-21-2010, 09:30 PM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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