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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by U17slash, 11-14-2022, 11:13 PM
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1 response
184 views
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by Lindsey
02-07-2023, 08:51 AM
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Started by samd199, 01-16-2023, 08:40 PM
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2 responses
288 views
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by samd199
01-20-2023, 11:24 AM
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Started by cey1001, 01-06-2023, 09:12 AM
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250 views
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by cey1001
01-06-2023, 09:12 AM
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Started by ASadiasa, 12-15-2022, 04:43 AM
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0 responses
133 views
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by ASadiasa
12-15-2022, 04:43 AM
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Started by egotistdown, 11-15-2021, 07:45 AM
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1 response
2,214 views
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Last Post
by xie186
11-22-2022, 06:33 PM
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Started by Conerx, 10-13-2022, 12:51 PM
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2 responses
461 views
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by Conerx
10-18-2022, 12:18 AM
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Started by GreenFrogs, 08-18-2022, 08:10 AM
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1 response
1,081 views
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by art_vandelay
10-10-2022, 03:22 PM
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Started by Sbiljh1, 11-30-2021, 08:26 AM
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1 response
1,762 views
0 reactions
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by Epimed
09-30-2022, 01:27 PM
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Started by nali, 09-27-2022, 04:38 PM
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0 responses
367 views
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Last Post
by nali
09-27-2022, 04:38 PM
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Started by preprep, 09-04-2022, 11:38 PM
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432 views
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Last Post
by preprep
09-04-2022, 11:38 PM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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Started by SEQadmin2, 07-02-2026, 11:08 AM
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07-02-2026, 11:08 AM
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