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We tried with a different flow cell and every thing was OK. The representitive company accepted the flowcell change. -
We had the same issue last week but with different LOTs. Did you solve the failure problem?Leave a comment:
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MiSeq Cycle 1 Possible Clustering Failure
We have run the same library on two different machines using a MiSeq V2 300 cycle kit and it has failed in cycle 1 both times. We are following the same protocol as we have for the last 6 years, which includes a 10% phiX control, and we are concerned this could be a LOT issue. Has anyone else had issues with Box 1 LOT 20778555 or Box 2 LOT 20774730?
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...10-30-2026, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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