Modern Methods and Techniques in Single-Cell Sequencing

Single-cell sequencing has become a transformative tool in biological research by providing scientists with the ability to detect cellular heterogeneity. The evolution of this technology has led to its widespread adoption, resulting in a seemingly endless list of publications, each emphasizing insights gained from its application. In this article, we’ll briefly review several key advancements in single-cell sequencing that have led to its continual rise.

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At the intersection of cytogenetics and genomics lies the exciting field of cytogenomics. It focuses on studying chromosomes at a molecular scale, involving techniques that analyze either the whole genome or particular DNA sequences to examine variations in structure and behavior at the chromosomal or subchromosomal level. By integrating cytogenetic techniques with genomic analysis, researchers can effectively investigate chromosomal abnormalities related to diseases, particularly...

Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...

Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.

Whole Transcriptome RNA-seq
Whole transcriptome sequencing...

Continual advancements in genomic and computational technologies have allowed researchers to construct precise pictures of individual genomes and identify variations that distinguish one genome from another. In order to fully understand the impact of these variants, it is essential to determine their chromosomal context in a process known as phasing. In this article, we'll explore some of the current technologies and approaches fundamental to this process.

What is ph...

San Diego-based Singular Genomics was founded in 2016 with the goal of overcoming the limitations of the available sequencing tools. Using feedback from scientists and clinicians, Singular designed and launched their G4ä Sequencing System. It stands out as a powerful benchtop sequencing platform with significantly reduced run times, delivering high-quality data in less than 24 hours across a wide array of applications. Notably, the G4 is capable of sequencing up to four human...

Plant genomes exhibit a remarkable range in size, stretching from incredibly compact genomes¹ to some of the largest known eukaryotic genomes². Significant progress has been made in understanding plant genomics since the first plant genome (Arabidopsis thaliana) was sequenced over 20 years ago³. Since that date, advances in sequencing technologies and analytical tools have expanded our knowledge, facilitating crop development, conservation efforts, and our understanding of...

Within the extensive array of instruments that Thermo Fisher Scientific offers, their Ion Torrent Next-Generation Sequencing systems remain important technologies for genomic research. Introduced in 2010 with the Ion PGM System (Personal Genome Machine), these sequencers have been continually evolving and facilitating new scientific insights.

After over a decade in the sequencing market, Jose Luis Costa, Global Director of Scientific Affairs at Thermo Fisher Scientific,...

Established in 2004, Pacific Biosciences (PacBio) set out to create innovative tools that would elevate our understanding of genomics. Over the years, they have grown into global leaders in the field, known for their revolutionary HiFi sequencing—a high-accuracy, long-read sequencing solution.

Their journey began with the PacBio RS, their first commercial release, which employed their Single Molecule, Real-Time (SMRT) sequencing technology. This marked the beginning...

After celebrating its 18^th birthday, the team at Complete Genomics is more committed than ever to providing the sequencing community with affordable sequencing solutions. It comes as no surprise as their Co-founder and Chief Scientific Officer Dr. Radoje (Rade) Drmanac has a long history of paving the way for the advancement of genomics. From working on the Human Genome Project to his various genomic startups, Drmanac has always had the goal of improving genomics research and technologi...