Metrics that Matter: Important Metrics for Long-Read Sequencing Experiments—Part 1

Long-read technologies have repeatedly demonstrated their value in genomics research. They’ve been used for improving genome assemblies¹, haplotype phasing², structural variant detection³, and epigenetic analysis⁴, among many other significant applications. When performing long-read sequencing experiments, it’s important to evaluate the success of the sequencing run using different metrics. Some metrics may be more relevant than others depending on the scope of the...

Welcome to part six of our Q&A article series with leading sequencing analysis providers. We’re interviewing these experts to gain helpful insights into their complex analysis processes.

In this final installment of our series, we ask our participants about one of the most important aspects of data analysis, accuracy and reproducibility.

If you’re just joining us, we recommend reviewing the first installment on quality control, the second installme...

This is part five of our Q&A article series with several leading sequencing analysis providers. We’re asking them important questions to learn how they handle different aspects of the analysis process.

In this latest segment of our series, we ask our participants about keeping up with the latest analysis trends.

Look back at the previous questions to review the first installment on quality control, the second installment on assemblies and alignments, the third...

This is part four of our Q&A article series with several leading sequencing analysis providers. We’re interviewing these experts to learn how they handle different aspects of the analysis process.

During this segment of our series, we ask the experts about the intricacies of data visualization.

In the first installment, we learned about quality control, the second installment covered assemblies and alignments, and the third installment focused on transcript...

This is part three of our Q&A article series with our expert sequencing analysis providers. We’re asking them important questions to learn how they handle different aspects of the analysis process.

In this segment of our series, we ask our participants about the complex processes of transcript quantification and differential expression analysis.

Review the first installment to see how they handle the quality control process, or the second installment to see...

This is part two of our Q&A article series where we ask several leading sequencing analysis providers for their approach to important analysis processes.

In this newest segment of our series, we ask our participants about their assessments of assemblies and alignments.

Take a look back at the first installment to review how each provider handles the quality control process of sequencing data. ...

As sequencing technologies and data analysis tools continue to advance, it is more important than ever to ensure your sequencing data is being handled appropriately. Analyzing sequencing data is a complex process and the current platforms are now capable of performing diverse tasks such as assembling genomes, quantifying transcripts, interpreting detailed experiments, and much more. In this Q&A article series, we’re interviewing top sequencing analysis providers to understand the working...

Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets....

Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches to targeted...

Using automation to prepare sequencing libraries isn’t a new concept, and most researchers are aware that there are numerous benefits to automating this process. However, many labs are still hesitant to switch to automation and often believe that it’s not suitable for their lab. To combat these concerns, we’ll cover some of the key advantages, review the most important considerations, and get real-world advice from automation experts to remove any lingering anxieties....