Does anyone know of any good methods for multiplexing more than 384 samples on a MiSeq run? The Nextera XT Index Kit allows indexing for a maximum of 384 samples. We have already purchased one of these kits, but would like to add more samples onto our run. I know the Illumina customer letter provided sequences for some of their barcodes, but not for all of them. Does anyone know if the rest of these are available elsewhere? I suppose it would be possible to order oligos identical to the Nextera barcode adaptors but with different 8 bp barcode sequences. Any thoughts?
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
