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  • genefuse
    Junior Member
    • Jan 2016
    • 7

    #1

    Tn5

    Does anyone know of another source of Tn5 transposase other than
    FC 121 1030 form Illumina.
    Cheers
  • Olaf Blue
    Member
    • Nov 2010
    • 58

    #2
    Epicentre's EZ-Tn5 Transposase; TNP92110, which is a special order item.

    Comment

    • genefuse
      Junior Member
      • Jan 2016
      • 7

      #3
      Thank you for the suggestion. Can Epicentre's EZ-Tn5 TNP92110 completely substitute for the Illumina product in terms of primer sequence, buffers, reaction conditions, etc.
      Appreciated,
      Genefuse

      Comment

      • Olaf Blue
        Member
        • Nov 2010
        • 58

        #4
        Happy to help - this is just the bare enzyme and users have to load any oligos themselves. Here's the product page link:



        Reaction buffer and stop solution is provided; we don't do custom oligo loading.

        Disclaimer: I am part of ILMN in Madison, WI

        Comment

        • genefuse
          Junior Member
          • Jan 2016
          • 7

          #5
          Great!
          Much appreciated
          Genefuse

          Comment

          • irene_chen
            Junior Member
            • Nov 2016
            • 2

            #6
            Hi, gene fuse
            Have you tried the EZ-Tn5 from epicenter? Could it be used for DNA library preparation?

            Comment

            • genefuse
              Junior Member
              • Jan 2016
              • 7

              #7
              Tn5

              Hi,
              I have not had a chance to try EN-Tn5.
              Olaf Blue might have more info on this
              Genefuse

              Comment

              • Olaf Blue
                Member
                • Nov 2010
                • 58

                #8
                EZ-Tn5 from Epicentre has been transferred for sales and support to Lucigen:

                A hyperactive form of the Tn5 transposase optimised for in vitro transposon mutagenesis (transposomics).


                Olaf

                Comment

                • irene_chen
                  Junior Member
                  • Nov 2016
                  • 2

                  #9
                  I am finding the replacement of Nextera from Illumina but I am not sure if it could be used for tagmentation. It seems to be mostly used for the gene mutation.

                  Comment

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