Tn5
Does anyone know of another source of Tn5 transposase other than
FC 121 1030 form Illumina.
Cheers
FC 121 1030 form Illumina.
Cheers
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Epicentre's EZ-Tn5 Transposase; TNP92110, which is a special order item. -
Thank you for the suggestion. Can Epicentre's EZ-Tn5 TNP92110 completely substitute for the Illumina product in terms of primer sequence, buffers, reaction conditions, etc.
Appreciated,
GenefuseComment
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Happy to help - this is just the bare enzyme and users have to load any oligos themselves. Here's the product page link:
Reaction buffer and stop solution is provided; we don't do custom oligo loading.
Disclaimer: I am part of ILMN in Madison, WIComment
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Great!
Much appreciated
GenefuseComment
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Hi, gene fuse
Have you tried the EZ-Tn5 from epicenter? Could it be used for DNA library preparation?Comment
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Tn5
Hi,
I have not had a chance to try EN-Tn5.
Olaf Blue might have more info on this
GenefuseComment
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EZ-Tn5 from Epicentre has been transferred for sales and support to Lucigen:
OlafComment
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I am finding the replacement of Nextera from Illumina but I am not sure if it could be used for tagmentation. It seems to be mostly used for the gene mutation.Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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