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Tn5
Does anyone know of another source of Tn5 transposase other than
FC 121 1030 form Illumina.
Cheers
FC 121 1030 form Illumina.
Cheers
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Epicentre's EZ-Tn5 Transposase; TNP92110, which is a special order item. -
Thank you for the suggestion. Can Epicentre's EZ-Tn5 TNP92110 completely substitute for the Illumina product in terms of primer sequence, buffers, reaction conditions, etc.
Appreciated,
GenefuseComment
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Happy to help - this is just the bare enzyme and users have to load any oligos themselves. Here's the product page link:
Reaction buffer and stop solution is provided; we don't do custom oligo loading.
Disclaimer: I am part of ILMN in Madison, WIComment
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Great!
Much appreciated
GenefuseComment
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Hi, gene fuse
Have you tried the EZ-Tn5 from epicenter? Could it be used for DNA library preparation?Comment
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Tn5
Hi,
I have not had a chance to try EN-Tn5.
Olaf Blue might have more info on this
GenefuseComment
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EZ-Tn5 from Epicentre has been transferred for sales and support to Lucigen:
OlafComment
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I am finding the replacement of Nextera from Illumina but I am not sure if it could be used for tagmentation. It seems to be mostly used for the gene mutation.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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