Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • genefuse
    Junior Member
    • Jan 2016
    • 7

    Tn5

    Does anyone know of another source of Tn5 transposase other than
    FC 121 1030 form Illumina.
    Cheers
  • Olaf Blue
    Member
    • Nov 2010
    • 58

    #2
    Epicentre's EZ-Tn5 Transposase; TNP92110, which is a special order item.

    Comment

    • genefuse
      Junior Member
      • Jan 2016
      • 7

      #3
      Thank you for the suggestion. Can Epicentre's EZ-Tn5 TNP92110 completely substitute for the Illumina product in terms of primer sequence, buffers, reaction conditions, etc.
      Appreciated,
      Genefuse

      Comment

      • Olaf Blue
        Member
        • Nov 2010
        • 58

        #4
        Happy to help - this is just the bare enzyme and users have to load any oligos themselves. Here's the product page link:



        Reaction buffer and stop solution is provided; we don't do custom oligo loading.

        Disclaimer: I am part of ILMN in Madison, WI

        Comment

        • genefuse
          Junior Member
          • Jan 2016
          • 7

          #5
          Great!
          Much appreciated
          Genefuse

          Comment

          • irene_chen
            Junior Member
            • Nov 2016
            • 2

            #6
            Hi, gene fuse
            Have you tried the EZ-Tn5 from epicenter? Could it be used for DNA library preparation?

            Comment

            • genefuse
              Junior Member
              • Jan 2016
              • 7

              #7
              Tn5

              Hi,
              I have not had a chance to try EN-Tn5.
              Olaf Blue might have more info on this
              Genefuse

              Comment

              • Olaf Blue
                Member
                • Nov 2010
                • 58

                #8
                EZ-Tn5 from Epicentre has been transferred for sales and support to Lucigen:

                A hyperactive form of the Tn5 transposase optimised for in vitro transposon mutagenesis (transposomics).


                Olaf

                Comment

                • irene_chen
                  Junior Member
                  • Nov 2016
                  • 2

                  #9
                  I am finding the replacement of Nextera from Illumina but I am not sure if it could be used for tagmentation. It seems to be mostly used for the gene mutation.

                  Comment

                  Latest Articles

                  Collapse

                  • SEQadmin2
                    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
                    by SEQadmin2


                    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


                    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
                    ...
                    06-02-2026, 10:05 AM
                  • SEQadmin2
                    Single-Cell Sequencing at an Inflection Point: Early Impacts of New Platforms and Emerging Trends
                    by SEQadmin2


                    With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.


                    Introduction

                    Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...
                    05-22-2026, 06:42 AM
                  • SEQadmin2
                    Environmental Genomics in the Age of NGS: From Microbes to Conservation Strategies
                    by SEQadmin2

                    Studying ecosystems means dealing with complex, multi-species communities that are hard to observe at scale. This complexity, however, hides many important questions to be answered, from how biogeochemical cycles work and how climate change can affect species distribution to how conservation strategies can work best.


                    Genomics, particularly since the expansion of NGS, has transformed ecosystem ecology. By sequencing environmental DNA, we can now assess biodiversity without direct...
                    05-06-2026, 09:04 AM

                  ad_right_rmr

                  Collapse

                  News

                  Collapse

                  Topics Statistics Last Post
                  Started by SEQadmin2, 06-02-2026, 12:03 PM
                  0 responses
                  20 views
                  0 reactions
                  Last Post SEQadmin2  
                  Started by SEQadmin2, 06-02-2026, 11:40 AM
                  0 responses
                  14 views
                  0 reactions
                  Last Post SEQadmin2  
                  Started by SEQadmin2, 05-28-2026, 11:40 AM
                  0 responses
                  29 views
                  0 reactions
                  Last Post SEQadmin2  
                  Started by SEQadmin2, 05-26-2026, 10:12 AM
                  0 responses
                  31 views
                  0 reactions
                  Last Post SEQadmin2  
                  Working...