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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by Fisale, 02-19-2025, 11:22 PM
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by Fisale
02-19-2025, 11:22 PM
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Started by User88, 01-21-2025, 05:18 PM
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1 response
624 views
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by fchatonnet
01-29-2025, 04:38 AM
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Started by gao gao, 12-25-2024, 07:11 AM
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200 views
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by gao gao
12-25-2024, 07:11 AM
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Started by AngelaT, 11-13-2024, 06:50 AM
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3 responses
275 views
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by fchatonnet
11-27-2024, 12:11 AM
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Started by mark_6060, 02-13-2023, 09:26 PM
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4 responses
1,159 views
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by AngelaT
11-13-2024, 08:00 AM
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Started by seleenjaber, 10-29-2024, 08:58 AM
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1 response
226 views
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by fchatonnet
11-13-2024, 01:21 AM
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Started by Ekaterina_Marnat, 09-27-2024, 12:13 AM
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1 response
289 views
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by fchatonnet
10-09-2024, 12:56 AM
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Started by Shia10, 09-04-2024, 07:17 AM
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0 responses
341 views
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by Shia10
09-04-2024, 07:17 AM
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Started by Praveenebenezer, 08-07-2024, 08:59 PM
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2 responses
379 views
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Started by zyt, 07-23-2024, 05:25 AM
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167 views
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by zyt
07-23-2024, 05:25 AM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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Started by SEQadmin2, Yesterday, 10:08 AM
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by SEQadmin2
Yesterday, 10:08 AM
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Started by SEQadmin2, 07-07-2026, 11:05 AM
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by SEQadmin2
07-07-2026, 11:05 AM
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Started by SEQadmin2, 07-02-2026, 11:08 AM
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by SEQadmin2
07-02-2026, 11:08 AM
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