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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by MMarti, 10-14-2013, 02:18 PM
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1 response
3,600 views
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by helico93561
11-10-2013, 06:33 PM
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Started by bensen, 10-24-2013, 05:08 AM
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2 responses
2,282 views
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by JeremyDay
11-08-2013, 04:11 PM
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Started by mgravina, 11-04-2013, 11:32 AM
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3 responses
4,638 views
0 reactions
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by JeremyDay
11-08-2013, 01:50 PM
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Started by Noa, 11-06-2013, 11:45 PM
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1 response
1,613 views
0 reactions
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by JeremyDay
11-08-2013, 01:37 PM
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Started by sinnafoch, 11-05-2013, 03:29 PM
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1 response
1,742 views
0 reactions
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by jwfoley
11-06-2013, 04:49 PM
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Started by cjang, 08-10-2012, 01:13 PM
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7 responses
6,030 views
0 reactions
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Last Post
by Genohub
11-05-2013, 02:08 PM
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Started by ymc, 11-04-2013, 11:50 PM
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1 response
1,837 views
0 reactions
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by gringer
11-05-2013, 12:09 AM
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Started by Smriti, 11-08-2011, 11:34 AM
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5 responses
9,109 views
0 reactions
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Last Post
by mmmm
11-02-2013, 09:57 AM
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Started by seqgirl123, 09-16-2010, 07:01 PM
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4 responses
10,848 views
0 reactions
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Last Post
by bstamps
11-02-2013, 07:06 AM
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Started by mdalessio, 10-31-2013, 12:44 PM
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2 responses
4,135 views
0 reactions
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Last Post
by bbeitzel
11-01-2013, 12:44 PM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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