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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by cmding, 02-23-2015, 06:56 PM
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by cmding
02-23-2015, 06:56 PM
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Started by Squall, 02-20-2015, 01:35 AM
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2 responses
1,268 views
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by Squall
02-23-2015, 12:35 AM
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Started by Irigoyen, 02-20-2015, 03:25 PM
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1 response
1,549 views
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Started by PhDstudent, 02-17-2015, 10:00 AM
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3 responses
1,497 views
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Started by libro.s, 02-02-2015, 02:30 PM
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2 responses
1,592 views
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by Hamid
02-19-2015, 11:10 AM
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Started by martinjf, 02-18-2015, 06:47 AM
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5 responses
2,536 views
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by martinjf
02-19-2015, 01:08 AM
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Started by herter, 02-15-2015, 07:09 AM
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1,238 views
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by herter
02-15-2015, 07:09 AM
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Started by 3gclone, 10-21-2014, 06:39 PM
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2 responses
3,646 views
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by VanessaS
02-13-2015, 03:17 PM
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Started by crimor, 07-11-2011, 08:22 AM
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3 responses
4,225 views
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by mcgarryg1
02-13-2015, 12:54 PM
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Started by amandasilveira, 02-13-2015, 12:57 AM
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1 response
1,209 views
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by HESmith
02-13-2015, 08:54 AM
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by SEQadmin2
Childhood cancers, effectively fatal 60 years ago, are now curable for up to 85% of children with access to contemporary treatments and...-
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05-08-2026, 01:42 AM -
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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Channel: Articles
Today, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
Channel: Articles
06-18-2026, 07:11 AM -
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by SEQadmin2
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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Channel: Articles
06-02-2026, 10:05 AM -
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Started by SEQadmin2, Yesterday, 05:37 AM
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by SEQadmin2
Yesterday, 05:37 AM
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Started by SEQadmin2, 06-26-2026, 11:10 AM
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by SEQadmin2
06-26-2026, 11:10 AM
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Whole-Genome Sequencing Traces Faroe Islands Ancestry to a North Atlantic Founder Population
by SEQadmin2
Started by SEQadmin2, 06-17-2026, 06:09 AM
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by SEQadmin2
06-17-2026, 06:09 AM
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