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Introductions
New here? Stop in and introduce yourself. Where you are, what you work on, etc.
Topics: 2,270 Posts: 4,389
2,270 4,389
Sequencing Resources
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Topics: 329 Posts: 797
329 797
News
News about the latest advances and discoveries in sequencing and related technologies.
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209 210
Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
Topics: 127 Posts: 577
127 577
General
Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
Topics: 2,606 Posts: 7,806
2,606 7,806
Webinar Series
Upcoming and on-demand webinars by SEQanswers and its partners.
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33 49
Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
Topics: 41,653 Posts: 42,332
41,653 42,332
Sequencing Technologies/Companies
Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 51 Posts: 160
51 160
Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
Topics: 1 Posts: 1
1 1
Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
Topics: 3,047 Posts: 16,700
3,047 16,700
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 391 Posts: 2,173
391 2,173
KlausR
by KlausR
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
Topics: 117 Posts: 594
117 594
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
Topics: 385 Posts: 2,176
385 2,176
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
Topics: 0 Posts: 0
0 0
Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
Topics: 0 Posts: 0
0 0
Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 76 Posts: 275
Last Post: SeqStudio users?
76 275
hamzairshad267
Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 79 Posts: 480
79 480
Archived Forums
A place for discussion of older forums and sequencing technologies.
Topics: 1,361 Posts: 7,278
Last Post: Rapid Libraries
1,361 7,278
pmiguel
by pmiguel
Sub-Forums:
UK - Cambridge (37/67)
The Pipeline (81/587)
SOLiD (462/2,440)
454 Pyrosequencing (743/3,966)
Polonator (6/65)
Bioinformatics
Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
Topics: 22,076 Posts: 103,043
22,076 103,043
Events / Conferences
Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
Topics: 767 Posts: 1,278
767 1,278
Jobs Forums
Industry Jobs!
Job listings at companies that sponsor SEQanswers.com! Interested in posting a job? Contact admin@ for access.
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222 253
vivianshaw
Academic/Non-Profit Jobs
Post and look for academic, non-profit, or other non-industrial jobs here.
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1,603 1,819
Applications Forums
Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,195 Posts: 9,880
2,195 9,880
Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
Topics: 6 Posts: 10
6 10
Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 278 Posts: 991
278 991
De novo discovery
Wandering without a reference? Post here
Topics: 341 Posts: 1,700
341 1,700
Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 273 Posts: 852
273 852
Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 357 Posts: 1,578
357 1,578
RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,866 Posts: 11,414
2,866 11,414
Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 42 Posts: 107
42 107
Personalized Genomics
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
Topics: 57 Posts: 290
57 290
bione
by bione
Site News
Site Announcements
Announcements regarding SEQanswers
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52 201
seqadmin
Site Feedback/Suggestions
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48 657

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    Current Approaches to Protein Sequencing


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
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    Techniques and Challenges in Conservation Genomics



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    03-08-2024, 10:41 AM

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